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Home>Product>Primary Antibody

CYP7B1 Mouse mAb

#3869

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Western blotting analysis using CYP7B1 antibody (Cat#3869). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYP7B1 antibody (Cat#3869, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with CYP7B1 antibody (Cat#3869, 1:500). Nuclei were stained blue with DAPI; CYP7B1 stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Flow cytometric analysis of CYP7B1 expression in HAP-1 cells using CYP7B1 antibody (Cat#3869, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CYP7B1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CYP7B1
  • Catalog：
    3869
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name CYP7B1 Mouse mAb
Aliases CYP7B1; Cytochrome P450 Family 7 Subfamily B Member 1; Cytochrome P450, Subfamily VIIB (Oxysterol 7 Alpha-Hydroxylase), Polypeptide 1; 25/26-Hydroxycholesterol 7-Alpha-Hydroxylase; 24-Hydroxycholesterol 7-Alpha-Hydroxylase; 3-Hydroxysteroid 7-Alpha Hydroxylase; Oxysterol 7-Alpha-Hydroxylase; Cytochrome P450 7B1; SPG5A; Cytochrome P450, Family 7, Subfamily B, Polypeptide 1; Spastic Paraplegia 5A (Autosomal Recessive); 25-Hydroxycholesterol 7-Alpha-Hydroxylase; EC 1.14.14.26; EC 1.14.14.29; CBAS3; CP7B
Background

Gene Name: CYP7B1

NCBI Gene Entry: 9420

UniProt Entry: O75881

Application Information

Molecular Weight: Predicted, 58 kDa; observed, 58 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB8275

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human CYP7B1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:200-1:1,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using CYP7B1 antibody (Cat#3869). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYP7B1 antibody (Cat#3869, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with CYP7B1 antibody (Cat#3869, 1:500). Nuclei were stained blue with DAPI; CYP7B1 stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
  • Flow cytometric analysis of CYP7B1 expression in HAP-1 cells using CYP7B1 antibody (Cat#3869, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CYP7B1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CYP7B1
  • Catalog：
    3869
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using CYP7B1 antibody (Cat#3869). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYP7B1 antibody (Cat#3869, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with CYP7B1 antibody (Cat#3869, 1:500). Nuclei were stained blue with DAPI; CYP7B1 stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar: 20 μm.
Flow cytometric analysis of CYP7B1 expression in HAP-1 cells using CYP7B1 antibody (Cat#3869, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CYP7B1.
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