Western blotting analysis using CHMP2B antibody (Cat#3917). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CHMP2B antibody (Cat#3917, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CHMP2B expression in HepG2 cells using CHMP2B antibody (Cat#3917, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CHMP2B.
CHMP2B; Charged Multivesicular Body Protein 2B; CHMP2.5; VPS2B; Charged Multivesicular Body Protein 2b; Chromatin Modifying Protein 2B; DKFZP564O123; Vacuolar Protein-Sorting-Associated Protein 2-2; Vacuolar Protein Sorting-Associated Protein 2-2; VPS2 Homolog B (S. Cerevisiae); Chromatin-Modifying Protein 2b; VPS2 Homolog B; FTDALS7; HVps2-2; VPS2-2; CHMP2b; Vps2-2; ALS17; DMT1
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from CHMP2B
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using CHMP2B antibody (Cat#3917). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CHMP2B antibody (Cat#3917, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CHMP2B expression in HepG2 cells using CHMP2B antibody (Cat#3917, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CHMP2B.
Western blotting analysis using CHMP2B antibody (Cat#3917). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CHMP2B antibody (Cat#3917, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CHMP2B expression in HepG2 cells using CHMP2B antibody (Cat#3917, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CHMP2B.
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
