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KD-Validated Podoplanin Recombinant Rabbit mAb

#62024

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using podoplanin antibody (Cat#62024). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with podoplanin antibody (Cat#62024, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using podoplanin antibody (Cat#62024). Podoplanin expression in wild-type (WT) and podoplanin (PDPN) shRNA knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with podoplanin antibody (Cat#62024, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PDPN
  • Catalog:
    62024
  • Application:
    WB
  • Species Reactivity:
    H, M
Information
Product Name KD-Validated Podoplanin Recombinant Rabbit mAb
Aliases PDPN; Podoplanin; Aggrus; PA2.26; T1A-2; D2-40; Gp38; GP40; Lung Type I Cell Membrane Associated Glycoprotein; Glycoprotein 36; PA2.26 Antigen;
T1-Alpha; GP36; T1A; Lung Type-I Cell Membrane-Associated Glycoprotein (T1A-2); Glycoprotein, 36-KD; HT1alpha-1; HT1alpha-2; HT1A-1; OTS8; T1A2;
TI1A; Gp36
Background

Gene Name: PDPN

NCBI Gene Entry: 10630

UniProt Entry: Q86YL7

Application Information

Molecular Weight: Predicted, 17 kDa; observed, 38 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB7870

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human Podoplanin
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using podoplanin antibody (Cat#62024). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with podoplanin antibody (Cat#62024, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using podoplanin antibody (Cat#62024). Podoplanin expression in wild-type (WT) and podoplanin (PDPN) shRNA knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with podoplanin antibody (Cat#62024, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using podoplanin antibody (Cat#62024). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with podoplanin antibody (Cat#62024, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using podoplanin antibody (Cat#62024). Podoplanin expression in wild-type (WT) and podoplanin (PDPN) shRNA knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with podoplanin antibody (Cat#62024, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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