Western blotting analysis using MMP9 antibody (Cat#6790). Total cell lysates (30 μg) from A549 cells (Lane 1: serum starved overnight, Lane 2: serum starved overnight, then treated with 80 nM PMA for 24 hours, then together with 1ug/ml BFA for another 3h) were loaded and separated by SDS-PAGE. The blot was incubated with MMP9 antibody (Cat#6790, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).