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Home>Product>Primary Antibody

NDUFS1 Recombinant Rabbit mAb

#6075

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Recombinant Antibody
Western blotting analysis using NDUFS1 antibody (Cat#6075). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFS1 antibody (Cat#6075, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human esophageal carcinoma using NDUFS1 antibody (Cat#6075, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse brain using NDUFS1 antibody (Cat#6075, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    NDUFS1
  • Catalog：
    6075
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name NDUFS1 Recombinant Rabbit mAb
Aliases NDUFS1; NADH:Ubiquinone Oxidoreductase Core Subunit S1; NADH-Ubiquinone Oxidoreductase 75 KDa Subunit, Mitochondrial; CI-75k; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 1, 75kDa (NADH-Coenzyme Q Reductase); Complex I 75kDa Subunit 2 3NADH Dehydrogenase (Ubiquinone) Fe-S Protein 1 (75kD) (NADH-Coenzyme Q Reductase); Mitochondrial NADH-Ubiquinone Oxidoreductase 75 KDa Subunit; Complex I, Mitochondrial Respiratory Chain, 75-KD Subunit; Complex I-75kD; EC 7.1.1.2; CI-75Kd; PRO1304; CI-75kD; MC1DN5
Background

Gene Name: NDUFS1

NCBI Gene Entry: 4719

UniProt Entry: P28331

Application Information

Molecular Weight: Predicted, 79 kDa; observed, 74 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB6700

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human Ndufs1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using NDUFS1 antibody (Cat#6075). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFS1 antibody (Cat#6075, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human esophageal carcinoma using NDUFS1 antibody (Cat#6075, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse brain using NDUFS1 antibody (Cat#6075, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NDUFS1
  • Catalog：
    6075
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using NDUFS1 antibody (Cat#6075). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFS1 antibody (Cat#6075, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human esophageal carcinoma using NDUFS1 antibody (Cat#6075, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse brain using NDUFS1 antibody (Cat#6075, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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