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Home>Product>Primary Antibody

KD-Validated FDFT1 Mouse mAb

#61778

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shRNA Knockdown Validated
Western blotting analysis using farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#61778). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#61778, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#61778). Farnesyl-diphosphate farnesyltransferase 1 expression in wild type (WT) and farnesyl-diphosphate farnesyltransferase 1 (FDFT1) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#61778, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Farnesyl-diphosphate farnesyltransferase 1 expression in HAP-1 cells using Farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#61778, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Farnesyl-diphosphate farnesyltransferase 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    FDFT1
  • Catalog：
    61778
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated FDFT1 Mouse mAb
Aliases FDFT1; Farnesyl-Diphosphate Farnesyltransferase 1; SQS; Squalene Synthase; FPP:FPP Farnesyltransferase; EC 2.5.1.21; SS; Farnesyl-Diphosphate Farnesyltransferase; Presqualene-Di-Diphosphate Synthase; Squalene Synthetase; DGPT; ERG9; SQSD
Background

Gene Name: FDFT1

NCBI Gene Entry: 2222

UniProt Entry: P37268

Application Information

Molecular Weight: Predicted, 48 kDa; observed, 48 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB3715

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human FDFT1
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#61778). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#61778, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#61778). Farnesyl-diphosphate farnesyltransferase 1 expression in wild type (WT) and farnesyl-diphosphate farnesyltransferase 1 (FDFT1) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#61778, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of Farnesyl-diphosphate farnesyltransferase 1 expression in HAP-1 cells using Farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#61778, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Farnesyl-diphosphate farnesyltransferase 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    FDFT1
  • Catalog：
    61778
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#61778). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#61778, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#61778). Farnesyl-diphosphate farnesyltransferase 1 expression in wild type (WT) and farnesyl-diphosphate farnesyltransferase 1 (FDFT1) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#61778, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Farnesyl-diphosphate farnesyltransferase 1 expression in HAP-1 cells using Farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#61778, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Farnesyl-diphosphate farnesyltransferase 1.
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