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Home>Product>Primary Antibody

COX IV Recombinant Rabbit mAb

#4057

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Recombinant Antibody
Western blotting analysis using COXIV antibody (Cat#4057). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with COXIV antibody (Cat#4057, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using COXIV antibody (Cat#4057, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of COXIV expression in HepG2 cells using COXIV antibody (Cat#4057, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, COXIV.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    COX4I1
  • Catalog：
    4057
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name COX IV Recombinant Rabbit mAb
Aliases COX4I1; Cytochrome C Oxidase Subunit 4I1; COXIV-1; COX4-1; COXIV; COX4; Cytochrome C Oxidase Subunit 4 Isoform 1, Mitochondrial; Cytochrome C Oxidase Subunit IV Isoform 1; Cytochrome C Oxidase Polypeptide IV; Cytochrome C Oxidase Subunit IV; COX IV-1; MC4DN16
Background

Gene Name: COX4I1

NCBI Gene Entry: 1327

UniProt Entry: P13073

Application Information

Molecular Weight: Predicted, 20 kDa; observed, 17 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB9375

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human COX IV
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using COXIV antibody (Cat#4057). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with COXIV antibody (Cat#4057, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using COXIV antibody (Cat#4057, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Flow cytometric analysis of COXIV expression in HepG2 cells using COXIV antibody (Cat#4057, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, COXIV.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    COX4I1
  • Catalog：
    4057
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using COXIV antibody (Cat#4057). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with COXIV antibody (Cat#4057, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using COXIV antibody (Cat#4057, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of COXIV expression in HepG2 cells using COXIV antibody (Cat#4057, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, COXIV.
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