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SPTLC1 Mouse mAb

#6380

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Western blotting analysis using SPTLC1 antibody (Cat#6380). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SPTLC1 antibody (Cat#6380, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of SPTLC1 expression in HAP-1 cells using SPTLC1 antibody (Cat#6380, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SPTLC1.
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Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    SPTLC1
  • Catalog:
    6380
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name SPTLC1 Mouse mAb
Aliases SPTLC1; Serine Palmitoyltransferase Long Chain Base Subunit 1; LCB1; HSAN1; SPTI; Long Chain Base Biosynthesis Protein 1; Serine-Palmitoyl-CoA Transferase 1; Serine Palmitoyltransferase 1; EC 2.3.1.50; HLCB1; HSN1; SPT1; Hereditary Sensory Neuropathy, Type 1; Serine C-Palmitoyltransferase; ALS27; LCB 1; SPT 1; LBC1
Background

Gene Name: SPTLC1

NCBI Gene Entry: 10558

UniProt Entry: O15269

Application Information

Molecular Weight: Predicted, 53 kDa; observed, 55 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB8775

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human SPTLC1
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:4,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using SPTLC1 antibody (Cat#6380). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SPTLC1 antibody (Cat#6380, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of SPTLC1 expression in HAP-1 cells using SPTLC1 antibody (Cat#6380, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SPTLC1.
Western blotting analysis using SPTLC1 antibody (Cat#6380). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SPTLC1 antibody (Cat#6380, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of SPTLC1 expression in HAP-1 cells using SPTLC1 antibody (Cat#6380, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SPTLC1.
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