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  • Primary Antibody
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Home>Product>Primary Antibody

Phospho-MEK1 (S298) Recombinant Rabbit mAb

#4227

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Recombinant Antibody
Western blotting analysis using phospho-MEK1 (S298) antibody (Cat#4227). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-MEK1 (S298) antibody (Cat#4227, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of phospho-MEK1 (S298) expression in C2C12 cells using phospho-MEK1 (S298) antibody (Cat#4227, 1:2,000). Green, isotype control; red, phospho-MEK1 (S298).
Immunocytochemical staining of C2C12 cells with phospho-MEK1 (S298) antibody (Cat#4227, 1:1,000). Nuclei were stained blue with DAPI; Phospho-MEK1 (S298) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    MAP2K1
  • Catalog：
    4227
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Phospho-MEK1 (S298) Recombinant Rabbit mAb
Aliases Mitogen-Activated Protein Kinase Kinase 1; MEK1; Dual Specificity Mitogen-Activated Protein Kinase Kinase 1; MAPK/ERK Kinase 1; MAPKK1; PRKMK1; MKK1; ERK Activator Kinase 1; MAP Kinase Kinase 1; EC 2.7.12.2; MAPKK 1; MEK 1; Protein Kinase, Mitogen-Activated, Kinase 1 (MAP Kinase Kinase 1); CFC3; MEL
Background

Gene Name: MAP2K1

NCBI Gene Entry: 5604

UniProt Entry: Q02750

Application Information

Molecular Weight: Predicted, 43 kDa, observed, 45 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10170

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-MEK1 (S298)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using phospho-MEK1 (S298) antibody (Cat#4227). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-MEK1 (S298) antibody (Cat#4227, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of phospho-MEK1 (S298) expression in C2C12 cells using phospho-MEK1 (S298) antibody (Cat#4227, 1:2,000). Green, isotype control; red, phospho-MEK1 (S298).
  • Immunocytochemical staining of C2C12 cells with phospho-MEK1 (S298) antibody (Cat#4227, 1:1,000). Nuclei were stained blue with DAPI; Phospho-MEK1 (S298) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    MAP2K1
  • Catalog：
    4227
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using phospho-MEK1 (S298) antibody (Cat#4227). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-MEK1 (S298) antibody (Cat#4227, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of phospho-MEK1 (S298) expression in C2C12 cells using phospho-MEK1 (S298) antibody (Cat#4227, 1:2,000). Green, isotype control; red, phospho-MEK1 (S298).
Immunocytochemical staining of C2C12 cells with phospho-MEK1 (S298) antibody (Cat#4227, 1:1,000). Nuclei were stained blue with DAPI; Phospho-MEK1 (S298) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

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  147. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Request.php ( 9.20 KB )
  148. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/mobile.php ( 4.08 KB )
  149. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Route.php ( 4.70 KB )
  150. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-multi-app/src/Url.php ( 7.52 KB )
  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
  154. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_cart.php ( 3.88 KB )
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  156. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/Response.php ( 8.81 KB )
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  158. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-trace/src/Html.php ( 4.42 KB )
  1. CONNECT:[ UseTime:0.000378s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000769s ]
  3. SELECT * FROM `goods` WHERE `code` = '4227' LIMIT 1 [ RunTime:0.000454s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000417s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000391s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000368s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 5559 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000336s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000502s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 5559 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000342s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000504s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000318s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.065946s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (6871,7463,3688,4422,10153,5499,8987,6536,5182,4604) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000728s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000484s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000202s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000568s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000248s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000314s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000455s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000248s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000250s ]
0.104809s