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Home>Product>Primary Antibody

DNA PKcs Recombinant Rabbit mAb

#4169

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Recombinant Antibody
Western blotting analysis using DNA PKcs antibody (Cat#4169). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNA PKcs antibody (Cat#4169, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using DNA PKcs antibody (Cat#4169, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with DNA PKcs antibody (Cat#4169, 1:1,000). Nuclei were stained blue with DAPI; DNA PKcs was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DNA PKcs expression in HepG2 cells using DNA PKcs antibody (Cat#4169, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DNA PKcs.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PRKDC
  • Catalog：
    4169
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name DNA PKcs Recombinant Rabbit mAb
Aliases PRKDC; Protein Kinase, DNA-Activated, Catalytic Subunit; DNPK1; P460; DNA-Dependent Protein Kinase Catalytic Subunit; DNA-PKcs; DNA-PKC; DNAPK; XRCC7; HYRC1; P350; HYRC; Protein Kinase, DNA-Activated, Catalytic Polypeptide; DNA-PK Catalytic Subunit; EC 2.7.11.1; DNAPKc; Hyper-Radiosensitivity Of Murine Scid Mutation, Complementing 1; DNA-PKCS; DNAPKC; IMD26
Background

Gene Name: PRKDC

NCBI Gene Entry: 5591

UniProt Entry: P78527

Application Information

Molecular Weight: Predicted, 469 kDa; observed, 469 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB9895

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human DNA-PKcs
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using DNA PKcs antibody (Cat#4169). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNA PKcs antibody (Cat#4169, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using DNA PKcs antibody (Cat#4169, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HepG2 cells with DNA PKcs antibody (Cat#4169, 1:1,000). Nuclei were stained blue with DAPI; DNA PKcs was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of DNA PKcs expression in HepG2 cells using DNA PKcs antibody (Cat#4169, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DNA PKcs.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PRKDC
  • Catalog：
    4169
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using DNA PKcs antibody (Cat#4169). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNA PKcs antibody (Cat#4169, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using DNA PKcs antibody (Cat#4169, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with DNA PKcs antibody (Cat#4169, 1:1,000). Nuclei were stained blue with DAPI; DNA PKcs was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DNA PKcs expression in HepG2 cells using DNA PKcs antibody (Cat#4169, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DNA PKcs.
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