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PSMA5 Recombinant Rabbit mAb

#5538

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Recombinant Antibody
Western blotting analysis using PSMA5 antibody (Cat#5538). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSMA5 antibody (Cat#5538, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PSMA5 expression in HepG2 cells using PSMA5 antibody (Cat#5538, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PSMA5.
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Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PSMA5
  • Catalog:
    5538
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name PSMA5 Recombinant Rabbit mAb
Aliases PSMA5; Proteasome 20S Subunit Alpha 5; ZETA; Proteasome (Prosome, Macropain) Subunit, Alpha Type, 5; Multicatalytic Endopeptidase Complex Zeta Chain; Proteasome Subunit Alpha Type-5; Proteasome Subunit Alpha 5; Proteasome Zeta Chain; Macropain Zeta Chain; Alpha-5; Epididymis Tissue Sperm Binding Protein Li 11n; Proteasome Alpha 5 Subunit; Proteasome Subunit Alpha-5; Proteasome Subunit Zeta; Macropain Subunit Zeta; Proteasome Component 5; EC 3.4.25.1; PSC5
Background

Gene Name: PSMA5

NCBI Gene Entry: 5686

UniProt Entry: P28066

Application Information

Molecular Weight: Predicted, 26 kDa; observed, 26 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB1970

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human PSMA5
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using PSMA5 antibody (Cat#5538). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSMA5 antibody (Cat#5538, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of PSMA5 expression in HepG2 cells using PSMA5 antibody (Cat#5538, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PSMA5.
Western blotting analysis using PSMA5 antibody (Cat#5538). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSMA5 antibody (Cat#5538, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PSMA5 expression in HepG2 cells using PSMA5 antibody (Cat#5538, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PSMA5.
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