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V5 Tag Recombinant Rabbit mAb

#T003

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Recombinant Antibody
Western blotting analysis using V5-tag antibody (Cat#T003). Recombinant multi-tag protein (5 ng) in E. coli were loaded and separated by SDS-PAGE. The blot was incubated with V5-tag antibody (Cat#T003, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
  • Catalog:
    T003
  • Application:
    WB
  • Species Reactivity:
    Recombinant protein
Information
Product Name V5 Tag Recombinant Rabbit mAb
Aliases V5-tag; GKPIPNPLLGLDST epitope tag; GKPIPNPLLGLDST tag; Protein Rev; Regulator of expression of viral proteins; rev; V5 epitope tag
Background The V5-tag is a short, artificial peptide sequence (GKPIPNPLLGLDST) derived from a small epitope present in the P and V proteins of the paramyxovirus simian virus 5 (SV5). It is genetically engineered and fused to either the N-terminus or C-terminus of a recombinant protein of interest, primarily serving as an epitope tag. Unlike affinity tags like the His-tag, its main purpose is not purification but rather highly specific detection and localization.
Application Information

Molecular Weight: Recombinant protein dependent

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB7075

Species Reactivity: Recombinant protein

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of V5 tag
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Note This product is for research use only.
Data
  • Western blotting analysis using V5-tag antibody (Cat#T003). Recombinant multi-tag protein (5 ng) in E. coli were loaded and separated by SDS-PAGE. The blot was incubated with V5-tag antibody (Cat#T003, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using V5-tag antibody (Cat#T003). Recombinant multi-tag protein (5 ng) in E. coli were loaded and separated by SDS-PAGE. The blot was incubated with V5-tag antibody (Cat#T003, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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