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Home>Product>Primary Antibody

KD-Validated Phospho-CDC6 (Ser106) Recombinant Rabbit mAb

#63263

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using phospho-CDC6 (Ser106) antibody (Cat#63263). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-CDC6 (Ser106) antibody (Cat#63263, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using phospho-CDC6 (Ser106) antibody (Cat#63263). Phospho-CDC6 (Ser106) expression in wild type (WT) and CDC6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-CDC6 (Ser106) antibody (Cat#63263, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). CDC6, cell division cycle 6.
Immunocytochemical staining of HepG2 cells with phospho-CDC6 (Ser106) antibody (Cat#63263, 1:1,000). Nuclei were stained blue with DAPI; Phospho-CDC6 (Ser106) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using Phospho-CDC6 (Ser106) antibody (Cat#63263, 1:1,000), Top panel: wild-type (WT); Bottom panal: Phospho-CDC6 (Ser106) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-CDC6 (Ser106) was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Validation of CDC6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phospho-CDC6 (Ser106) antibody (Cat#63263, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CDC6
  • Catalog：
    63263
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated Phospho-CDC6 (Ser106) Recombinant Rabbit mAb
Aliases CDC6; Cell Division Cycle 6; CDC18L; Cell Division Control Protein 6 Homolog; Cdc18-Related Protein; CDC6-Related Protein; P62(Cdc6); HsCDC6; CDC6 (Cell Division Cycle 6, S. Cerevisiae) Homolog; CDC6 Cell Division Cycle 6 Homolog (S. Cerevisiae); Cell Division Cycle 6 Homolog (S. Cerevisiae); CDC6 Cell Division Cycle 6 Homolog; Cell Division Cycle 6 Homolog; HsCDC18; HsCdc18; MGORS5
Background

Gene Name: CDC6

NCBI Gene Entry: 990

UniProt Entry: Q99741

Application Information

Molecular Weight: Predicted, 63 kDa; observed, 63 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4770

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-CDC6 (Ser106)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using phospho-CDC6 (Ser106) antibody (Cat#63263). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-CDC6 (Ser106) antibody (Cat#63263, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using phospho-CDC6 (Ser106) antibody (Cat#63263). Phospho-CDC6 (Ser106) expression in wild type (WT) and CDC6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-CDC6 (Ser106) antibody (Cat#63263, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). CDC6, cell division cycle 6.
  • Immunocytochemical staining of HepG2 cells with phospho-CDC6 (Ser106) antibody (Cat#63263, 1:1,000). Nuclei were stained blue with DAPI; Phospho-CDC6 (Ser106) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Immunocytochemical staining of HeLa cells using Phospho-CDC6 (Ser106) antibody (Cat#63263, 1:1,000), Top panel: wild-type (WT); Bottom panal: Phospho-CDC6 (Ser106) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-CDC6 (Ser106) was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Validation of CDC6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phospho-CDC6 (Ser106) antibody (Cat#63263, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CDC6
  • Catalog：
    63263
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using phospho-CDC6 (Ser106) antibody (Cat#63263). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-CDC6 (Ser106) antibody (Cat#63263, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using phospho-CDC6 (Ser106) antibody (Cat#63263). Phospho-CDC6 (Ser106) expression in wild type (WT) and CDC6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-CDC6 (Ser106) antibody (Cat#63263, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). CDC6, cell division cycle 6.
Immunocytochemical staining of HepG2 cells with phospho-CDC6 (Ser106) antibody (Cat#63263, 1:1,000). Nuclei were stained blue with DAPI; Phospho-CDC6 (Ser106) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HeLa cells using Phospho-CDC6 (Ser106) antibody (Cat#63263, 1:1,000), Top panel: wild-type (WT); Bottom panal: Phospho-CDC6 (Ser106) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-CDC6 (Ser106) was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Validation of CDC6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phospho-CDC6 (Ser106) antibody (Cat#63263, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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