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Home>Product>Primary Antibody

KRT6A Mouse mAb

#5073

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Western blotting analysis using keratin 6A antibody (Cat#5073). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 6A antibody (Cat#5073, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HaCaT cells with Keratin 6A antibody (Cat#5073, 1:1,000). Nuclei were stained blue with DAPI; Keratin 6A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Keratin 6A expression in HaCaT cells using Keratin 6A antibody (Cat#5073, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Keratin 6A.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    KRT6A
  • Catalog：
    5073
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name KRT6A Mouse mAb
Aliases KRT6A; Keratin 6A; KRT6D; CK6C; CK6D; K6C; K6D; Keratin, Type II Cytoskeletal 6A; Keratin 6A, Type II; ype-II Keratin Kb6; KRT6C; K6A; Keratin, Epidermal Type II, K6A; Keratin 6A, , Type II; Allergen Hom S 5; Cytokeratin 6A; Cytokeratin 6C; Cytokeratin 6D; Cytokeratin-6A; Cytokeratin-6D; Keratin 6C; Keratin 6D; Keratin-6A; CK-6C; CK-6E; CK-6A; CK-6D; CK6A; PC3
Background

Gene Name: KRT6A

NCBI Gene Entry: 3853

UniProt Entry: P02538

Application Information

Molecular Weight: Predicted, 60 kDa; observed, 52 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB14180

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human KRT6A
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using keratin 6A antibody (Cat#5073). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 6A antibody (Cat#5073, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HaCaT cells with Keratin 6A antibody (Cat#5073, 1:1,000). Nuclei were stained blue with DAPI; Keratin 6A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Keratin 6A expression in HaCaT cells using Keratin 6A antibody (Cat#5073, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Keratin 6A.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    KRT6A
  • Catalog：
    5073
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using keratin 6A antibody (Cat#5073). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 6A antibody (Cat#5073, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HaCaT cells with Keratin 6A antibody (Cat#5073, 1:1,000). Nuclei were stained blue with DAPI; Keratin 6A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Keratin 6A expression in HaCaT cells using Keratin 6A antibody (Cat#5073, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Keratin 6A.
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