Western blotting analysis using adiponectin receptor 1 antibody (Cat#4196). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with adiponectin receptor 1 antibody (Cat#4196, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Adiponectin receptor 1 antibody (Cat#4196, 1:1,000). Nuclei were stained blue with DAPI; Adiponectin receptor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of adiponectin receptor 1 expression in C2C12 cells using adiponectin receptor 1 antibody (Cat#4196, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, adiponectin receptor 1.
ADIPOR1; Adiponectin Receptor 1; PAQR1; ACDCR1; Progestin And AdipoQ Receptor Family Member 1; Progestin And AdipoQ Receptor Family Member I; Adiponectin Receptor Protein 1; TESBP1A; CGI-45; CGI45
Western blotting analysis using adiponectin receptor 1 antibody (Cat#4196). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with adiponectin receptor 1 antibody (Cat#4196, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Adiponectin receptor 1 antibody (Cat#4196, 1:1,000). Nuclei were stained blue with DAPI; Adiponectin receptor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of adiponectin receptor 1 expression in C2C12 cells using adiponectin receptor 1 antibody (Cat#4196, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, adiponectin receptor 1.
Western blotting analysis using adiponectin receptor 1 antibody (Cat#4196). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with adiponectin receptor 1 antibody (Cat#4196, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Adiponectin receptor 1 antibody (Cat#4196, 1:1,000). Nuclei were stained blue with DAPI; Adiponectin receptor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of adiponectin receptor 1 expression in C2C12 cells using adiponectin receptor 1 antibody (Cat#4196, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, adiponectin receptor 1.
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