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Home>Product>Primary Antibody

Histone Deacetylase 9 Recombinant Rabbit mAb

#4238

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Recombinant Antibody
Western blotting analysis using histone deacetylase 9 antibody (Cat#4238). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with histone deacetylase 9 antibody (Cat#4238, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Histone deacetylase 9 antibody (Cat#4238, 1:1,000). Nuclei were stained blue with DAPI; Histone deacetylase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of histone deacetylase 9 expression in HepG2 cells using histone deacetylase 9 antibody (Cat#4238, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, histone deacetylase 9.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HDAC9
  • Catalog：
    4238
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name Histone Deacetylase 9 Recombinant Rabbit mAb
Aliases HDAC9; Histone Deacetylase 9; HDAC7B; MITR; HD7; KIAA0744; HDAC; Histone Deacetylase 7B; EC 3.5.1.98; HDAC; HD7b; HDRP; HD9; MEF-2 Interacting Transcription Repressor (MITR) Protein; MEF2-Interacting Transcription Repressor MITR; Histone Deacetylase 4/5-Related Protein; Histone Deacetylase-Related Protein; HDAC9FL; ARCND4; HDAC9B
Background

Gene Name: HDAC9

NCBI Gene Entry: 9734

UniProt Entry: Q9UKV0

Application Information

Molecular Weight: Predicted, 111 kDa; observed, 160 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10225

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human HDAC9
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:4,000-1:20,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using histone deacetylase 9 antibody (Cat#4238). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with histone deacetylase 9 antibody (Cat#4238, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with Histone deacetylase 9 antibody (Cat#4238, 1:1,000). Nuclei were stained blue with DAPI; Histone deacetylase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of histone deacetylase 9 expression in HepG2 cells using histone deacetylase 9 antibody (Cat#4238, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, histone deacetylase 9.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HDAC9
  • Catalog：
    4238
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using histone deacetylase 9 antibody (Cat#4238). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with histone deacetylase 9 antibody (Cat#4238, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Histone deacetylase 9 antibody (Cat#4238, 1:1,000). Nuclei were stained blue with DAPI; Histone deacetylase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of histone deacetylase 9 expression in HepG2 cells using histone deacetylase 9 antibody (Cat#4238, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, histone deacetylase 9.
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