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Home>Product>Primary Antibody

KD-Validated RAD51 Mouse mAb

#62075

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shRNA Knockdown Validated
Western blotting analysis using RAD51 antibody (Cat#62075). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAD51 antibody (Cat#62075, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using RAD51 antibody (Cat#62075). RAD51 expression in wild-type (WT) and RAD51 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RAD51 antibody (Cat#62075, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RAD51
  • Catalog：
    62075
  • Application：
    WB
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated RAD51 Mouse mAb
Aliases RAD51; RAD51 Recombinase; HsT16930; HsRad51; RAD51A; BRCC5; FANCR; RECA; BRCA1/BRCA2-Containing Complex, Subunit 5; DNA Repair Protein RAD51 Homolog 1; RAD51 Homolog A; HRAD51; RAD51 Homolog (RecA Homolog, E. Coli) (S. Cerevisiae); RAD51 (S. Cerevisiae) Homolog (E Coli RecA Homolog); RAD51 Homolog (S. Cerevisiae); RecA, E. Coli, Homolog Of; Recombination Protein A; RecA-Like Protein; HsRAD51; MRMV2
Background

Gene Name: RAD51

NCBI Gene Entry: 5888

UniProt Entry: Q06609

Application Information

Molecular Weight: Predicted, 37 kDa; observed, 36 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9800

Species Reactivity: Human, mouse, rat

Applications Tested: Western Blotting (WB)
Immunogen Recombinant protein of human RAD51
Isotype Mouse IgG2b
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using RAD51 antibody (Cat#62075). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAD51 antibody (Cat#62075, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using RAD51 antibody (Cat#62075). RAD51 expression in wild-type (WT) and RAD51 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RAD51 antibody (Cat#62075, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RAD51
  • Catalog：
    62075
  • Application：
    WB
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using RAD51 antibody (Cat#62075). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAD51 antibody (Cat#62075, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using RAD51 antibody (Cat#62075). RAD51 expression in wild-type (WT) and RAD51 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RAD51 antibody (Cat#62075, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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