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Home>Product>Primary Antibody

KO-Validated Catenin Beta 1 Mouse mAb

#71274

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Knockout Validated
Western blotting analysis using catenin beta 1 antibody (Cat#71274). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with catenin beta 1 antibody (Cat#71274, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using catenin beta 1 antibody (Cat#71274). Catenin beta 1 expression in wild-type (WT) and catenin beta 1 (CTNNB1) knockout (KO) 293T cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with catenin beta 1 antibody (Cat#71274, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Catenin beta 1 expression in HAP-1 cells using Catenin beta 1 antibody (Cat#71274, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Catenin beta 1.
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Quantity：
1
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  • Gene Symbol：
    CTNNB1
  • Catalog：
    71274
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KO-Validated Catenin Beta 1 Mouse mAb
Aliases CTNNB1; Catenin Beta 1; Beta-Catenin; Armadillo; CTNNB; Catenin (Cadherin-Associated Protein), Beta 1, 88kDa; Catenin Beta-1; Catenin (Cadherin-Associated Protein), Beta 1 (88kD); Catenin (Cadherin-Associated Protein), Beta 1; NEDSDV; MRD19; EVR7
Background

Gene Name: CTNNB1

NCBI Gene Entry: 1499

UniProt Entry: P35222

Application Information

Molecular Weight: Predicted, 85 kDa; observed, 85 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB5850

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human CTNNB1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using catenin beta 1 antibody (Cat#71274). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with catenin beta 1 antibody (Cat#71274, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using catenin beta 1 antibody (Cat#71274). Catenin beta 1 expression in wild-type (WT) and catenin beta 1 (CTNNB1) knockout (KO) 293T cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with catenin beta 1 antibody (Cat#71274, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of Catenin beta 1 expression in HAP-1 cells using Catenin beta 1 antibody (Cat#71274, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Catenin beta 1.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CTNNB1
  • Catalog：
    71274
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using catenin beta 1 antibody (Cat#71274). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with catenin beta 1 antibody (Cat#71274, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using catenin beta 1 antibody (Cat#71274). Catenin beta 1 expression in wild-type (WT) and catenin beta 1 (CTNNB1) knockout (KO) 293T cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with catenin beta 1 antibody (Cat#71274, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Catenin beta 1 expression in HAP-1 cells using Catenin beta 1 antibody (Cat#71274, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Catenin beta 1.
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