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Home>Product>Primary Antibody

ILF3 Recombinant Rabbit mAb

#3018

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Recombinant Antibody
Western blotting analysis using Interleukin enhancer binding factor 3 antibody (Cat#3018). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Interleukin enhancer binding factor 3 antibody (Cat#3018, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with interleukin enhancer binding factor 3 antibody (Cat#3018, 1:1,000). Nuclei were stained blue with DAPI; Interleukin enhancer binding factor 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Interleukin enhancer binding factor 3 expression in HepG2 cells using Interleukin enhancer binding factor 3 antibody (Cat#3018, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Interleukin enhancer binding factor 3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ILF3
  • Catalog：
    3018
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name ILF3 Recombinant Rabbit mAb
Aliases ILF3; Interleukin Enhancer Binding Factor 3; MPHOSPH4; DRBP76; NF90; MPP4; Nuclear Factor Of Activated T-Cells 90 KDa; Translational Control Protein 80; M-Phase Phosphoprotein 4; NFAR110; NF90ctv; MPP4110; NFAR-1; NFAR-2; TCP110; NFAR90; NF110b; NF110; NF90a; NF90c; M Phase Phosphoprotein 4, Nuclear Factor Associated With DS RNA; Interleukin Enhancer Binding Factor 3, 90kDa; Nuclear Factor Of Activated T-Cells 110 KDa; Interleukin Enhancer Binding Factor 3, 90kD; Double-Stranded RNA-Binding Protein, 76 KD; Interleukin Enhancer-Binding Factor 3; Nuclear Factor Associated With DsRNA; DsRNA Binding Protein NFAR-2/MPP4; NF-AT-90; TCP80; DRBF; NFAR; Nuclear Factor Of Activated T-Cells, 90 KD; Double-Stranded RNA-Binding Protein 76; NF90b; NFAR2; CBTF; MMP4
Background

Gene Name: ILF3

NCBI Gene Entry: 3609

UniProt Entry: Q12906

Application Information

Molecular Weight: Predicted, 95 kDa; observed, 110 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3655

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human ILF3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Interleukin enhancer binding factor 3 antibody (Cat#3018). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Interleukin enhancer binding factor 3 antibody (Cat#3018, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with interleukin enhancer binding factor 3 antibody (Cat#3018, 1:1,000). Nuclei were stained blue with DAPI; Interleukin enhancer binding factor 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Interleukin enhancer binding factor 3 expression in HepG2 cells using Interleukin enhancer binding factor 3 antibody (Cat#3018, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Interleukin enhancer binding factor 3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ILF3
  • Catalog：
    3018
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Interleukin enhancer binding factor 3 antibody (Cat#3018). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Interleukin enhancer binding factor 3 antibody (Cat#3018, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with interleukin enhancer binding factor 3 antibody (Cat#3018, 1:1,000). Nuclei were stained blue with DAPI; Interleukin enhancer binding factor 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Interleukin enhancer binding factor 3 expression in HepG2 cells using Interleukin enhancer binding factor 3 antibody (Cat#3018, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Interleukin enhancer binding factor 3.
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