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CD116 Recombinant Rabbit mAb

#2253

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Recombinant Antibody
Western blotting analysis using CD116 antibody (Cat#2253). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD116 antibody (Cat#2253, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CD116 expression in HepG2 cells using CD116 antibody (Cat#2253, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CD116.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    CSF2RA
  • Catalog:
    2253
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name CD116 Recombinant Rabbit mAb
Aliases CSF2RA; Colony Stimulating Factor 2 Receptor Subunit Alpha; CD116; CSF2R; Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage); Granulocyte-Macrophage Colony-Stimulating Factor Receptor Subunit Alpha; Alpha-GM-CSF Receptor; GM-CSF-R-Alpha; CD116 Antigen; GMCSFR-Alpha; GMR-Alpha; AlphaGMR; CDw116; CSF2RY; Granulocyte-Macrophage Colony-Stimulating Factor Receptor Alpha Chain; Colony Stimulating Factor 2 Receptor Alpha Subunit; GM-CSF Receptor Alpha Subunit; ALPHAGMR; CSF2RAX; CSF2RAY; CSF2RX; GMCSFR; SMDP4; GMR
Background

Gene Name: CSF2RA

NCBI Gene Entry: 1438

UniProt Entry: P15509

Application Information

Molecular Weight: Predicted, 46 kDa; observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5695

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human CD116
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using CD116 antibody (Cat#2253). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD116 antibody (Cat#2253, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of CD116 expression in HepG2 cells using CD116 antibody (Cat#2253, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CD116.
Western blotting analysis using CD116 antibody (Cat#2253). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD116 antibody (Cat#2253, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CD116 expression in HepG2 cells using CD116 antibody (Cat#2253, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CD116.
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