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Keratin 5 Recombinant Rabbit mAb

#2126

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Recombinant Antibody
Western blotting analysis using Keratin 5 antibody (Cat#2126). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Keratin 5 antibody (Cat#2126, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Keratin 5 expression in C2C12 cells using Keratin 5 antibody (Cat#2126, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Keratin 5.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    KRT5
  • Catalog:
    2126
  • Application:
    WB, FCM
  • Species Reactivity:
    M, R
Information
Product Name Keratin 5 Recombinant Rabbit mAb
Aliases KRT5; Keratin 5; CK-5; KRT5A; Keratin 5 (Epidermolysis Bullosa Simplex, Dowling-Meara/Kobner/Weber-Cockayne Types); Epidermolysis Bullosa Simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types; Keratin, Type II Cytoskeletal 5; Type-II Keratin Kb5; Keratin 5, Type II; Cytokeratin-5; EBS2; K5; 58 Kda Cytokeratin; 58 KDa Cytokeratin; Keratin-5; EBS2A; EBS2B; EBS2C; EBS2D; EBS2E; EBS2F; DDD1; EBS1; CK5; DDD
Background

Gene Name: KRT5

NCBI Gene Entry: 3852

UniProt Entry: P13647

Application Information

Molecular Weight: Predicted, 62 kDa; observed, 62 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB6120

Species Reactivity: Mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human Cytokeratin 5
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using Keratin 5 antibody (Cat#2126). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Keratin 5 antibody (Cat#2126, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Keratin 5 expression in C2C12 cells using Keratin 5 antibody (Cat#2126, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Keratin 5.
Western blotting analysis using Keratin 5 antibody (Cat#2126). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Keratin 5 antibody (Cat#2126, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Keratin 5 expression in C2C12 cells using Keratin 5 antibody (Cat#2126, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Keratin 5.
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