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Home>Product>Primary Antibody

Collagen Type III Alpha 1 Chain Mouse mAb

#3489

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Western blotting analysis using COL3A1 antibody (Cat#3489). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with COL3A1 antibody (Cat#3489, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716). COL3A1, collagen type III alpha 1 chain.
Immunocytochemical staining of H9C2 cells with COL3A1 antibody (Cat#3489, 1:1,000). Nuclei were stained blue with DAPI;COL3A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of COL3A1 expression in H9c2 cells using COL3A1 antibody (Cat#3489, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, COL3A1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    COL3A1
  • Catalog：
    3489
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    M, R

Information

Data

Information
Product Name Collagen Type III Alpha 1 Chain Mouse mAb
Aliases Collagen Type III Alpha 1 Chain; Ehlers-Danlos Syndrome Type IV, Autosomal Dominant; Collagen, Type III, Alpha; Collagen Alpha-1(III) Chain; EDS4A; Alpha-1 Type III Collagen; Alpha1 (III) Collagen; Collagen, Fetal; EDSVASC; PMGEDSV
Background

Gene Name: COL3A1

NCBI Gene Entry: 1281

UniProt Entry: P02461

Application Information

Molecular Weight: Predicted, 139 kDa; observed, 150 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB5870

Species Reactivity: Mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human COL3A1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB):1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using COL3A1 antibody (Cat#3489). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with COL3A1 antibody (Cat#3489, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716). COL3A1, collagen type III alpha 1 chain.
  • Immunocytochemical staining of H9C2 cells with COL3A1 antibody (Cat#3489, 1:1,000). Nuclei were stained blue with DAPI;COL3A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of COL3A1 expression in H9c2 cells using COL3A1 antibody (Cat#3489, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, COL3A1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    COL3A1
  • Catalog：
    3489
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    M, R
RELATED PRODUCTS
Western blotting analysis using COL3A1 antibody (Cat#3489). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with COL3A1 antibody (Cat#3489, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716). COL3A1, collagen type III alpha 1 chain.
Immunocytochemical staining of H9C2 cells with COL3A1 antibody (Cat#3489, 1:1,000). Nuclei were stained blue with DAPI;COL3A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of COL3A1 expression in H9c2 cells using COL3A1 antibody (Cat#3489, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, COL3A1.
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