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SDHB Recombinant Rabbit mAb

#2571

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Recombinant Antibody
Western blotting analysis using SDHB antibody (Cat#2571). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SDHB antibody (Cat#2571, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of SDHB expression in C2C12 cells using SDHB antibody (Cat#2571, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SDHB.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    SDHB
  • Catalog:
    2571
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name SDHB Recombinant Rabbit mAb
Aliases SDHB; Succinate Dehydrogenase Complex Iron Sulfur Subunit B; Iron-Sulfur Subunit Of Complex II; SDH1; SDH; Succinate Dehydrogenase [Ubiquinone] Iron-Sulfur Subunit, Mitochondrial; Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip); Succinate Dehydrogenase Complex Subunit B, Iron Sulfur (Ip); Succinate Dehydrogenase [Ubiquinone] Iron-Sulfur Subunit; EC 1.3.5.1; MC2DN4; PPGL4; SDHIP; CWS2; PGL4; SDH2; IP; Ip
Background

Gene Name: SDHB

NCBI Gene Entry: 6390

UniProt Entry: P21912

Application Information

Molecular Weight: Predicted, 32 kDa; observed, 32 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1455

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human SDHB
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using SDHB antibody (Cat#2571). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SDHB antibody (Cat#2571, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of SDHB expression in C2C12 cells using SDHB antibody (Cat#2571, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SDHB.
Western blotting analysis using SDHB antibody (Cat#2571). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SDHB antibody (Cat#2571, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of SDHB expression in C2C12 cells using SDHB antibody (Cat#2571, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SDHB.
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