Western blotting analysis using MRI1 antibody (Cat#65829). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MRI1 antibody (Cat#65829, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using MRI1 antibody (Cat#65829). MRI1 expression in wild-type (WT) and MRI1 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MRI1 antibody (Cat#65829, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using MRI1 antibody (Cat#65829, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse liver using MRI1 antibody (Cat#65829, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunocytochemical staining of HAP-1 cells with MRI1 antibody(Cat#65829, 1:1,000) . Nuclei were stained blue with DAPI; MRI1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using MRI1 antibody (Cat#65829, 1:1,000), Top panel: wild-type (WT); Bottom panal: MRI1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; MRI1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Flow cytometric analysis of MRI1 expression in HAP-1 cells using MRI1 antibody (Cat#65829, 1:2,000). Green, isotype control; red, MRI1.
Validation of MRI1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with MRI1 antibody (Cat#65829, 1:2,000) and analyzed using BD flow cytometer.
