Western blotting analysis using MRPL11 antibody (Cat#5706). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MRPL11 antibody (Cat#5706, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with MRPL11 antibody (Cat#5706, 1:1,000). Nuclei were stained blue with DAPI; MRPL11 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
MRPL11; Mitochondrial Ribosomal Protein L11; UL11m; 39S Ribosomal Protein L11, Mitochondrial; Large Ribosomal Subunit Protein UL11m; MRP-L11; Mitochondrial Large Ribosomal Subunit Protein UL11m; CGI-113; L11MT; L11mt
Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen
Recombinant protein of human MRPL11
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Immunocytochemistry (ICC): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using MRPL11 antibody (Cat#5706). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MRPL11 antibody (Cat#5706, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with MRPL11 antibody (Cat#5706, 1:1,000). Nuclei were stained blue with DAPI; MRPL11 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using MRPL11 antibody (Cat#5706). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MRPL11 antibody (Cat#5706, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with MRPL11 antibody (Cat#5706, 1:1,000). Nuclei were stained blue with DAPI; MRPL11 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
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