Western blotting analysis using HSPA1A antibody (Cat#6575). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSPA1A antibody (Cat#6575, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using HSPA1A antibody (Cat#6575). Total cell lysates (30 μg) from H9c2 and C2C12 cells were loaded and separated by SDS-PAGE. The blot was incubated with HSPA1A antibody (Cat#6575, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with HSPA1A antibody (Cat#6575, 1:1,000). Nuclei were stained blue with DAPI; HSPA1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar, 20 μm.
Flow cytometric analysis of HSPA1A expression in HepG2 cells using HSPA1A antibody (Cat#6575, 1:2,000). Green, isotype control; red, HSPA1A.
