Western blotting analysis using ASCC2 antibody (Cat#5337). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ASCC2 antibody (Cat#5337, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of ASCC2 expression in HepG2 cells using ASCC2 antibody (Cat#5337, 1:2,000). Green, isotype control; red, ASCC2.
Immunocytochemical staining of HepG2 cells with ASCC2 antibody (Cat#5337, 1:1,000). Nuclei were stained blue with DAPI; ASCC2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Low. Scale bar, 20 μm.
Western blotting analysis using ASCC2 antibody (Cat#5337). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ASCC2 antibody (Cat#5337, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of ASCC2 expression in HepG2 cells using ASCC2 antibody (Cat#5337, 1:2,000). Green, isotype control; red, ASCC2.
Immunocytochemical staining of HepG2 cells with ASCC2 antibody (Cat#5337, 1:1,000). Nuclei were stained blue with DAPI; ASCC2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Low. Scale bar, 20 μm.
Western blotting analysis using ASCC2 antibody (Cat#5337). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ASCC2 antibody (Cat#5337, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of ASCC2 expression in HepG2 cells using ASCC2 antibody (Cat#5337, 1:2,000). Green, isotype control; red, ASCC2.
Immunocytochemical staining of HepG2 cells with ASCC2 antibody (Cat#5337, 1:1,000). Nuclei were stained blue with DAPI; ASCC2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Low. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.002811s ]
SELECT * FROM `goods` WHERE `code` = '5337' LIMIT 1 [ RunTime:0.000519s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.002801s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000373s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.001489s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6425 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000389s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000942s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6425 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000739s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000977s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000565s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.106767s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (6804,9062,5448,8449,7179,5665,5907,7285,5858,7167) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000962s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000515s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000248s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000487s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000226s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000331s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000422s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000254s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000225s ]
