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MCM7 Recombinant Rabbit mAb

#4371

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Recombinant Antibody
Western blotting analysis using MCM7 antibody (Cat#4371). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MCM7 antibody (Cat#4371, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with MCM7 antibody (Cat#4371, 1:1,000). Nuclei were stained blue with DAPI; MCM7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of MCM7 expression in HepG2 cells using MCM7 antibody (Cat#4371, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, MCM7.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    MCM7
  • Catalog:
    4371
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, R
Information
Product Name MCM7 Recombinant Rabbit mAb
Aliases Minichromosome Maintenance Complex Component 7; CDC47; PPP1R104; MCM2; Protein Phosphatase 1, Regulatory Subunit 104; DNA Replication Licensing Factor MCM7; CDC47 Homolog; P1.1-MCM3; MCM7 Minichromosome Maintenance Deficient 7 (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 7; Minichromosome Maintenance Deficient 7; Homolog Of S. Cerevisiae Cdc47; EC 3.6.4.12; P1CDC47; PNAS146; P85MCM
Background

Gene Name: MCM7

NCBI Gene Entry: 4176

UniProt Entry: P33993

Application Information

Molecular Weight: Predicted, 81 kDa; observed, 81 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10885

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human MCM7
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using MCM7 antibody (Cat#4371). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MCM7 antibody (Cat#4371, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with MCM7 antibody (Cat#4371, 1:1,000). Nuclei were stained blue with DAPI; MCM7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of MCM7 expression in HepG2 cells using MCM7 antibody (Cat#4371, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, MCM7.
Western blotting analysis using MCM7 antibody (Cat#4371). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MCM7 antibody (Cat#4371, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with MCM7 antibody (Cat#4371, 1:1,000). Nuclei were stained blue with DAPI; MCM7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of MCM7 expression in HepG2 cells using MCM7 antibody (Cat#4371, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, MCM7.
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