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Home>Product>Primary Antibody

Lysine Demethylase 1B Recombinant Rabbit mAb

#4376

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Recombinant Antibody
Western blotting analysis using lysine demethylase 1B antibody (Cat#4376). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with lysine demethylase 1B antibody (Cat#4376, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with lysine demethylase 1B antibody (Cat#4376, 1:1,000). Nuclei were stained blue with DAPI; Lysine demethylase 1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of lysine demethylase 1B expression in HepG2 cells using lysine demethylase 1B antibody (Cat#4376, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, lysine demethylase 1B.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    KDM1B
  • Catalog：
    4376
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Lysine Demethylase 1B Recombinant Rabbit mAb
Aliases KDM1B; Lysine Demethylase 1B; LSD2; AOF1; Flavin-Containing Amine Oxidase Domain-Containing Protein 1; Lysine-Specific Histone Demethylase 1B; Lysine-Specific Histone Demethylase; Lysine (K)-Specific Demethylase 1B; DJ298J15.2; BA204B7.3; FLJ34109; FLJ33898; FLJ43328; C6orf193; Amine Oxidase (Flavin Containing) Domain 1; Chromosome 6 Open Reading Frame 193; Amine Oxidase, Flavin Containing 1; EC 1.14.99.66; C6ORF193
Background

Gene Name: KDM1B

NCBI Gene Entry: 221656

UniProt Entry: Q8NB78

Application Information

Molecular Weight: Predicted, 92 kDa; observed, 75 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10910

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human LSD2 / AOF1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using lysine demethylase 1B antibody (Cat#4376). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with lysine demethylase 1B antibody (Cat#4376, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with lysine demethylase 1B antibody (Cat#4376, 1:1,000). Nuclei were stained blue with DAPI; Lysine demethylase 1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of lysine demethylase 1B expression in HepG2 cells using lysine demethylase 1B antibody (Cat#4376, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, lysine demethylase 1B.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    KDM1B
  • Catalog：
    4376
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using lysine demethylase 1B antibody (Cat#4376). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with lysine demethylase 1B antibody (Cat#4376, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with lysine demethylase 1B antibody (Cat#4376, 1:1,000). Nuclei were stained blue with DAPI; Lysine demethylase 1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of lysine demethylase 1B expression in HepG2 cells using lysine demethylase 1B antibody (Cat#4376, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, lysine demethylase 1B.
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