Western blotting analysis using ACP5 antibody (Cat#1738). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ACP5 antibody (Cat#1738, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with ACP5 antibody (Cat#1738, 1:1,000). Nuclei were stained blue with DAPI; ACP5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using ACP5 antibody (Cat#1738, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Applications Tested: Western blotting (WB), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen
A synthesized peptide derived from human Tartrate Resistant Acid Phosphatase
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (ICC): 1:100-1:1,000 Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note
This product is for research use only.
Data
Western blotting analysis using ACP5 antibody (Cat#1738). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ACP5 antibody (Cat#1738, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with ACP5 antibody (Cat#1738, 1:1,000). Nuclei were stained blue with DAPI; ACP5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using ACP5 antibody (Cat#1738, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Western blotting analysis using ACP5 antibody (Cat#1738). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ACP5 antibody (Cat#1738, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with ACP5 antibody (Cat#1738, 1:1,000). Nuclei were stained blue with DAPI; ACP5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using ACP5 antibody (Cat#1738, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
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