Western blotting analysis using WDR47 antibody (Cat#6304). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WDR47 antibody (Cat#6304, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HACAT cells with WDR47 antibody (Cat#6304, 1:1,000) . Nuclei were stained blue with DAPI; WDR47 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Flow cytometric analysis of WDR47 expression in HaCaT cells using WDR47 antibody (Cat#6304,1:2,000). Green, isotype control; red, WDR47.