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PFKM Recombinant Rabbit mAb

#4465

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Recombinant Antibody
Western blotting analysis using PFKM antibody (Cat#4465). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PFKM antibody (Cat#4465, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PFKM expression in HepG2 cells using PFKM antibody (Cat#4465, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PFKM.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PFKM
  • Catalog:
    4465
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name PFKM Recombinant Rabbit mAb
Aliases PFKM; Phosphofructokinase, Muscle; PPP1R122; PFK-1; PFKX; ATP-Dependent 6-Phosphofructokinase, Muscle Type; Protein Phosphatase 1, Regulatory Subunit 122; Phosphofructokinase, Polypeptide X; Phosphofructo-1-Kinase Isozyme A; 6-Phosphofructokinase Type A; Phosphohexokinase; EC 2.7.1.11; ATP-PFK; PFK-A; 6-Phosphofructokinase, Muscle Type; 6-Phosphofructo-1-Kinase; Phosphofructokinase 1; Phosphofructokinase-M; EC 2.7.1; PFK-M; GSD7; PFK1; PFKA
Background

Gene Name: PFKM

NCBI Gene Entry: 5213

UniProt Entry: P08237

Application Information

Molecular Weight: Predicted, 85 kDa; observed, 75 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11355

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human Fructose 6 Phosphate Kinase
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using PFKM antibody (Cat#4465). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PFKM antibody (Cat#4465, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of PFKM expression in HepG2 cells using PFKM antibody (Cat#4465, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PFKM.
Western blotting analysis using PFKM antibody (Cat#4465). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PFKM antibody (Cat#4465, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PFKM expression in HepG2 cells using PFKM antibody (Cat#4465, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PFKM.
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