Western blotting analysis using NCK1 antibody (Cat#2408). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NCK1 antibody (Cat#2408, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with NCK1 antibody (Cat#2408, 1:1,000). Nuclei were stained blue with DAPI; NCK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of NCK1 expression in HT-1080 cells using NCK1 antibody (Cat#2408, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, NCK1.
NCK Adaptor Protein 1; NCKalpha; NCK; SH2/SH3 Adaptor Protein NCK-Alpha; Cytoplasmic Protein NCK1; Nck-1; Non-Catalytic Region Of Tyrosine Kinase; Melanoma NCK Protein; NCK Tyrosine Kinase
Western blotting analysis using NCK1 antibody (Cat#2408). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NCK1 antibody (Cat#2408, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with NCK1 antibody (Cat#2408, 1:1,000). Nuclei were stained blue with DAPI; NCK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of NCK1 expression in HT-1080 cells using NCK1 antibody (Cat#2408, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, NCK1.
Western blotting analysis using NCK1 antibody (Cat#2408). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NCK1 antibody (Cat#2408, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with NCK1 antibody (Cat#2408, 1:1,000). Nuclei were stained blue with DAPI; NCK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of NCK1 expression in HT-1080 cells using NCK1 antibody (Cat#2408, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, NCK1.
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