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Home>Product>Primary Antibody

Complement C3 Recombinant Rabbit mAb

#4535

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Recombinant Antibody
Western blotting analysis using complement C3 antibody (Cat#4535). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with complement C3 antibody (Cat#4535, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells with Complement C3 antibody (Cat#4535, 1:1,000). Nuclei were stained blue with DAPI; Complement C3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    C3
  • Catalog：
    4535
  • Application：
    WB, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name Complement C3 Recombinant Rabbit mAb
Aliases C1QBP; Complement C3; CPAMD1; ARMD9; C3 And PZP-Like Alpha-2-Macroglobulin Domain-Containing Protein 1; Complement Component C3a; Complement Component C3b; Complement Component 3; C3a Anaphylatoxin; Prepro-C3; C3a; C3b; Epididymis Secretory Sperm Binding Protein Li 62p; Acylation-Stimulating Protein Cleavage Product; HEL-S-62p; AHUS5; ASP; C3A; C3B
Background

Gene Name: C3

NCBI Gene Entry: 718

UniProt Entry: P01024

Application Information

Molecular Weight: Predicted, 187 kDa; observed, 187,115 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11705

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human C3 / C3a
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using complement C3 antibody (Cat#4535). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with complement C3 antibody (Cat#4535, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HeLa cells with Complement C3 antibody (Cat#4535, 1:1,000). Nuclei were stained blue with DAPI; Complement C3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    C3
  • Catalog：
    4535
  • Application：
    WB, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using complement C3 antibody (Cat#4535). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with complement C3 antibody (Cat#4535, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells with Complement C3 antibody (Cat#4535, 1:1,000). Nuclei were stained blue with DAPI; Complement C3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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