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Home>Product>Primary Antibody

APBB1 Recombinant Rabbit mAb

#4545

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Recombinant Antibody
Western blotting analysis using APBB1 antibody (Cat#4545). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with APBB1 antibody (Cat#4545, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with APBB1 antibody (Cat#4545, 1:1,000). Nuclei were stained blue with DAPI; APBB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of APBB1 expression in HAP-1 cells using APBB1 antibody (Cat#4545, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, APBB1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    APBB1
  • Catalog：
    4545
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name APBB1 Recombinant Rabbit mAb
Aliases APBB1; Amyloid Beta Precursor Protein Binding Family B Member 1; FE65; RIR; Amyloid Beta (A4) Precursor Protein-Binding, Family B, Member 1 (Fe65); Amyloid-Beta A4 Precursor Protein-Binding Family B Member 1; Adaptor Protein FE65a2; Stat-Like Protein; Protein Fe65; MGC:9072; Fe65
Background

Gene Name: APBB1

NCBI Gene Entry: 322

UniProt Entry: O00213

Application Information

Molecular Weight: Predicted, 77 kDa; observed, 100 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11755

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human FE65
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using APBB1 antibody (Cat#4545). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with APBB1 antibody (Cat#4545, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with APBB1 antibody (Cat#4545, 1:1,000). Nuclei were stained blue with DAPI; APBB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of APBB1 expression in HAP-1 cells using APBB1 antibody (Cat#4545, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, APBB1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    APBB1
  • Catalog：
    4545
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using APBB1 antibody (Cat#4545). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with APBB1 antibody (Cat#4545, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with APBB1 antibody (Cat#4545, 1:1,000). Nuclei were stained blue with DAPI; APBB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of APBB1 expression in HAP-1 cells using APBB1 antibody (Cat#4545, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, APBB1.
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