Western blotting analysis using Arp2 antibody (Cat#4597). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Arp2 antibody (Cat#4597, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Arp2 antibody(Cat#4597, 1:1,000). Nuclei were stained blue with DAPI; Arp2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Arp2 expression in C2C12 cells using Arp2 antibody (Cat#4597, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Arp2.
ACTR2; Actin Related Protein 2; ARP2; ARP2 Actin Related Protein 2 Homolog; Actin-Related Protein 2; Actin-Like Protein 2; ARP2 (Actin-Related Protein 2, Yeast) Homolog; ARP2 Actin-Related Protein 2 Homolog (Yeast)
Western blotting analysis using Arp2 antibody (Cat#4597). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Arp2 antibody (Cat#4597, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Arp2 antibody(Cat#4597, 1:1,000). Nuclei were stained blue with DAPI; Arp2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Arp2 expression in C2C12 cells using Arp2 antibody (Cat#4597, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Arp2.
Western blotting analysis using Arp2 antibody (Cat#4597). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Arp2 antibody (Cat#4597, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Arp2 antibody(Cat#4597, 1:1,000). Nuclei were stained blue with DAPI; Arp2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Arp2 expression in C2C12 cells using Arp2 antibody (Cat#4597, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Arp2.
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