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Home>Product>Primary Antibody

Doublecortin Like Kinase 1 Recombinant Rabbit mAb

#4709

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Recombinant Antibody
Western blotting analysis using doublecortin like kinase 1 antibody (Cat#4709). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with doublecortin like kinase 1 antibody (Cat#4709, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Doublecortin like kinase 1 antibody ((Cat#4709, 1:1,000). Nuclei were stained blue with DAPI; Doublecortin like kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Doublecortin like kinase 1 expression in C2C12 cells using Doublecortin like kinase 1 antibody ((Cat#4709, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Doublecortin like kinase 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DCLK1
  • Catalog：
    4709
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M

Information

Data

Information
Product Name Doublecortin Like Kinase 1 Recombinant Rabbit mAb
Aliases DCLK1; Doublecortin Like Kinase 1; DCDC3A; KIAA0369; DCAMKL1; DCLK; Doublecortin Domain-Containing Protein 3A; Doublecortin-Like And CAM Kinase-Like 1; Serine/Threonine-Protein Kinase DCLK1; Doublecortin-Like Kinase 1; EC 2.7.11.1; Doublecortin And CaM Kinase-Like 1; EC 2.7.11; CLICK1; CL1
Background

Gene Name: DCLK1

NCBI Gene Entry: 9201

UniProt Entry: O15075

Application Information

Molecular Weight: Predicted, 82 kDa; observed, 75 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB12555

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human DCAMKL1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using doublecortin like kinase 1 antibody (Cat#4709). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with doublecortin like kinase 1 antibody (Cat#4709, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with Doublecortin like kinase 1 antibody ((Cat#4709, 1:1,000). Nuclei were stained blue with DAPI; Doublecortin like kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Doublecortin like kinase 1 expression in C2C12 cells using Doublecortin like kinase 1 antibody ((Cat#4709, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Doublecortin like kinase 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DCLK1
  • Catalog：
    4709
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M
RELATED PRODUCTS
Western blotting analysis using doublecortin like kinase 1 antibody (Cat#4709). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with doublecortin like kinase 1 antibody (Cat#4709, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Doublecortin like kinase 1 antibody ((Cat#4709, 1:1,000). Nuclei were stained blue with DAPI; Doublecortin like kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Doublecortin like kinase 1 expression in C2C12 cells using Doublecortin like kinase 1 antibody ((Cat#4709, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Doublecortin like kinase 1.
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