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EIF3B Recombinant Rabbit mAb

#4713

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Recombinant Antibody
Western blotting analysis using EIF3B antibody (Cat#4713). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF3B antibody (Cat#4713, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of EIF3B expression in HepG2 cells using EIF3B antibody (Cat#4713, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, EIF3B.
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Specification:
Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    EIF3B
  • Catalog:
    4713
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name EIF3B Recombinant Rabbit mAb
Aliases EIF3B; Eukaryotic Translation Initiation Factor 3 Subunit B; EIF3S9; PRT1; Eukaryotic Translation Initiation Factor 3, Subunit 9 Eta, 116kDa; Eukaryotic Translation Initiation Factor 3 Subunit 9; Protein Synthesis 1; Prt1 Homolog; Eukaryotic Translation Initiation Factor 3, Subunit 9 (Eta, 116kD); Eukaryotic Translation Initiation Factor 3, Subunit B; EIF3-P110; EIF3-P116; EIF-3-Eta; EIF3 P110; EIF3 P116; EIF3-ETA; EIF3b; HPrt1
Background

Gene Name: EIF3B

NCBI Gene Entry: 8662

UniProt Entry: P55884

Application Information

Molecular Weight: Predicted, 92 kDa; observed, 117 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB12575

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human eIF3B
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using EIF3B antibody (Cat#4713). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF3B antibody (Cat#4713, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of EIF3B expression in HepG2 cells using EIF3B antibody (Cat#4713, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, EIF3B.
Western blotting analysis using EIF3B antibody (Cat#4713). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF3B antibody (Cat#4713, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of EIF3B expression in HepG2 cells using EIF3B antibody (Cat#4713, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, EIF3B.
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