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Caspase 9 Recombinant Rabbit mAb

#4755

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Recombinant Antibody
Western blotting analysis using caspase 9 antibody (Cat#4755). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caspase 9 antibody (Cat#4755, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Caspase 9 expression in HepG2 cells using Caspase 9 antibody (Cat#4755, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Caspase 9.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    CASP9
  • Catalog:
    4755
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name Caspase 9 Recombinant Rabbit mAb
Aliases CASP9; Caspase 9; ICE-LAP6; APAF-3; MCH6; PPP1R56; Caspase 9, Apoptosis-Related Cysteine Peptidase; Protein Phosphatase 1, Regulatory Subunit 56; ICE-Like Apoptotic Protease 6; Caspase-9; Caspase 9, Apoptosis-Related Cysteine Protease; Apoptotic Protease Activating Factor 3; Apoptotic Protease-Activating Factor 3; Apoptotic Protease MCH-6; Apoptotic Protease Mch-6; EC 3.4.22.62; CASP-9; APAF3
Background

Gene Name: CASP9

NCBI Gene Entry: 842

UniProt Entry: P55211

Application Information

Molecular Weight: Predicted, 46 kDa; observed, 46 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB12780

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human Caspase-9
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using caspase 9 antibody (Cat#4755). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caspase 9 antibody (Cat#4755, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Caspase 9 expression in HepG2 cells using Caspase 9 antibody (Cat#4755, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Caspase 9.
Western blotting analysis using caspase 9 antibody (Cat#4755). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caspase 9 antibody (Cat#4755, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Caspase 9 expression in HepG2 cells using Caspase 9 antibody (Cat#4755, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Caspase 9.
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