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GTF3C4 Mouse mAb

#4833

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Western blotting analysis using GTF3C4 antibody (Cat#4833). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GTF3C4 antibody (Cat#4833, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with GTF3C4 antibody (Cat#4833, 1:1,000). Nuclei were stained blue with DAPI; GTF3C4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of GTF3C4 expression in HepG2 cells using GTF3C4 antibody (Cat#4833, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, GTF3C4.
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Unit price:
$69.00
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1
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  • Gene Symbol:
    GTF3C4
  • Catalog:
    4833
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H
Information
Product Name GTF3C4 Mouse mAb
Aliases GTF3C4; General Transcription Factor IIIC Subunit 4; TFIIIC90; KAT12; General Transcription Factor IIIC, Polypeptide 4, 90kDa; General Transcription Factor 3C Polypeptide 4; Transcription Factor IIIC 90 KDa Subunit; Transcription Factor IIIC Subunit Delta; TFIIIC 90 KDa Subunit; TF3C-Delta; General Transcription Factor IIIC, Polypeptide 4 (90kD); General Transcription Factor 3C 4; TFIIICDELTA; EC 2.3.1.48; TFIIIC290; TFIII90
Background

Gene Name: GTF3C4

NCBI Gene Entry: 9329

UniProt Entry: Q9UKN8

Application Information

Molecular Weight: Predicted, 92 kDa; observed, 80 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB13170

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen Recombinant protein of human GTF3C4
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:200-1:1,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using GTF3C4 antibody (Cat#4833). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GTF3C4 antibody (Cat#4833, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with GTF3C4 antibody (Cat#4833, 1:1,000). Nuclei were stained blue with DAPI; GTF3C4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of GTF3C4 expression in HepG2 cells using GTF3C4 antibody (Cat#4833, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, GTF3C4.
Western blotting analysis using GTF3C4 antibody (Cat#4833). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GTF3C4 antibody (Cat#4833, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with GTF3C4 antibody (Cat#4833, 1:1,000). Nuclei were stained blue with DAPI; GTF3C4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of GTF3C4 expression in HepG2 cells using GTF3C4 antibody (Cat#4833, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, GTF3C4.
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