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Home>Product>Primary Antibody

POLR2E Mouse mAb

#4845

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Western blotting analysis using POLR2E antibody (Cat#4845). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with POLR2E antibody (Cat#4845, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of POLR2E expression in HAP-1 cells using POLR2E antibody (Cat#4845, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, POLR2E.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    POLR2E
  • Catalog：
    4845
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name POLR2E Mouse mAb
Aliases POLR2E; RNA Polymerase II, I And III Subunit E; XAP4; RPABC1; HRPB25; HsRPB5; RPB5; DNA-Directed RNA Polymerases I, II, And III Subunit RPABC1; DNA Directed RNA Polymerase II 23 Kda Polypeptide; DNA-Directed RNA Polymerase II 23 KDa Polypeptide; RNA Polymerases I, II, And III Subunit ABC1; DNA-Directed RNA Polymerase II Subunit E; RNA Polymerase II Subunit E; RPB5 Homolog; Polymerase (RNA) II (DNA Directed) Polypeptide E (25kD); Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa; DNA-Directed RNA Polymerase Subunit RPABC1; Polymerase (RNA) II Subunit E
Background

Gene Name: POLR2E

NCBI Gene Entry: 5434

UniProt Entry: P19388

Application Information

Molecular Weight: Predicted, 25 kDa; observed, 25 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB13230

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human POLR2E
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using POLR2E antibody (Cat#4845). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with POLR2E antibody (Cat#4845, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of POLR2E expression in HAP-1 cells using POLR2E antibody (Cat#4845, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, POLR2E.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    POLR2E
  • Catalog：
    4845
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using POLR2E antibody (Cat#4845). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with POLR2E antibody (Cat#4845, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of POLR2E expression in HAP-1 cells using POLR2E antibody (Cat#4845, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, POLR2E.
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