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KD-Validated KAT2A Recombinant Rabbit mAb

#67021

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using KAT2A antibody (Cat#67021, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using KAT2A antibody (Cat#67021, 1:1,000). KAT2A expression in wild-type (WT) and KAT2A shRNA knockdown (KD) HeLa cells of total cell lysates. GAPDH serves as a loading control.
Western blotting analysis using KAT2A antibody (Cat#67021). Total cell lysates (40 μg) from various cell lines and mouse heart tissue were loaded and separated by SDS-PAGE. The blot was incubated with KAT2A antibody (Cat#67021, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:10,000) respectively. Image was developed using PiQ™ ECL Substrate Kit (Cat#636).
Immunocytochemical staining of HeLa cells using KAT2A antibody (Cat#67021, 1:100). Nuclei were stained blue with DAPI; β-actin (ACTB) was stained green with Alexa Flour® 488; KAT2A was stained magenta with Alexa Flour® 647.
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  • Gene Symbol:
    KAT2A
  • Catalog:
    67021
  • Application:
    WB, ICC
  • Species Reactivity:
    H, M, R, Mk
Information
Product Name KD-Validated KAT2A Recombinant Rabbit mAb
Aliases KAT2A; Lysine Acetyltransferase 2A; GCN5; PCAF-B; GCN5L2; General Control Of Amino Acid Synthesis Protein 5-Like 2; Histone Glutaryltransferase KAT2A; Histone Succinyltransferase KAT2A; Histone Acetyltransferase KAT2A; K(Lysine) Acetyltransferase 2A; Histone Acetyltransferase GCN5; EC 2.3.1.48; STAF97; HGCN5; GCN5 (General Control Of Amino-Acid Synthesis, Yeast, Homolog)-Like 2; General Control Of Amino Acid Synthesis, Yeast, Homolog-Like 2; GCN5 General Control Of Amino-Acid Synthesis 5-Like 2 (Yeast); EC 2.3.1.-; HsGCN5
Background

Gene Name: KAT2A

NCBI Gene Entry: 2648

UniProt Entry: Q92830

Application Information

Molecular Weight: Predicted, 94 kDa; observed, 94 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB2425

Species Reactivity: Human, mouse, rat, monkey

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human KAT2A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000
Immunocytochemistry (ICC): 1:100
Note This product is for research use only.
Data
  • Western blotting analysis using KAT2A antibody (Cat#67021, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
  • Western blotting analysis using KAT2A antibody (Cat#67021, 1:1,000). KAT2A expression in wild-type (WT) and KAT2A shRNA knockdown (KD) HeLa cells of total cell lysates. GAPDH serves as a loading control.
  • Western blotting analysis using KAT2A antibody (Cat#67021). Total cell lysates (40 μg) from various cell lines and mouse heart tissue were loaded and separated by SDS-PAGE. The blot was incubated with KAT2A antibody (Cat#67021, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:10,000) respectively. Image was developed using PiQ™ ECL Substrate Kit (Cat#636).
  • Immunocytochemical staining of HeLa cells using KAT2A antibody (Cat#67021, 1:100). Nuclei were stained blue with DAPI; β-actin (ACTB) was stained green with Alexa Flour® 488; KAT2A was stained magenta with Alexa Flour® 647.
Western blotting analysis using KAT2A antibody (Cat#67021, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using KAT2A antibody (Cat#67021, 1:1,000). KAT2A expression in wild-type (WT) and KAT2A shRNA knockdown (KD) HeLa cells of total cell lysates. GAPDH serves as a loading control.
Western blotting analysis using KAT2A antibody (Cat#67021). Total cell lysates (40 μg) from various cell lines and mouse heart tissue were loaded and separated by SDS-PAGE. The blot was incubated with KAT2A antibody (Cat#67021, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:10,000) respectively. Image was developed using PiQ™ ECL Substrate Kit (Cat#636).
Immunocytochemical staining of HeLa cells using KAT2A antibody (Cat#67021, 1:100). Nuclei were stained blue with DAPI; β-actin (ACTB) was stained green with Alexa Flour® 488; KAT2A was stained magenta with Alexa Flour® 647.
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