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Home>Product>Primary Antibody

KD-Validated Catenin Delta 1 Mouse mAb

#63468

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shRNA Knockdown Validated
Western blotting analysis using catenin delta 1 antibody (Cat#63468). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with catenin delta 1 antibody (Cat#63468, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Western blotting analysis using catenin delta 1 antibody (Cat#63468). Catenin delta 1 expression in wild type (WT) and catenin delta 1 (CTNND1) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with catenin delta 1 antibody (Cat#63468, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using Catenin delta 1 antibody (Cat#63468, 1:1,000), Top panel: wild-type (WT); Bottom panal: Catenin delta 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Catenin delta 1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of H9C2 cells with Catenin delta 1 antibody(Cat #63468, 1:1,000). Nuclei were stained blue with DAPI; Catenin delta 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar: 20 μm.
Flow cytometric analysis of Catenin delta 1 expression in H9c2 cells using Catenin delta 1 antibody (Cat#63468, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Catenin delta 1.
Validation of Catenin delta 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Catenin delta 1 antibody (Cat#63468, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CTNND1
  • Catalog：
    63468
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated Catenin Delta 1 Mouse mAb
Aliases CTNND1; Catenin Delta 1; KIAA0384; P120; Catenin (Cadherin-Associated Protein), Delta 1; Cadherin-Associated Src Substrate; Catenin Delta-1; P120 Catenin; P120CAS; P120CTN; CTNND; CAS; P120(CAS); P120(CTN); P120(Ctn); P120(Cas); P120cas; P120ctn; BCDS2
Background

Gene Name: CTNND1

NCBI Gene Entry: 1500

UniProt Entry: O60716

Application Information

Molecular Weight: Predicted, 108 kDa; observed, 108 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB5775

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human CTNND1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB):1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using catenin delta 1 antibody (Cat#63468). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with catenin delta 1 antibody (Cat#63468, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
  • Western blotting analysis using catenin delta 1 antibody (Cat#63468). Catenin delta 1 expression in wild type (WT) and catenin delta 1 (CTNND1) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with catenin delta 1 antibody (Cat#63468, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HeLa cells using Catenin delta 1 antibody (Cat#63468, 1:1,000), Top panel: wild-type (WT); Bottom panal: Catenin delta 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Catenin delta 1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Immunocytochemical staining of H9C2 cells with Catenin delta 1 antibody(Cat #63468, 1:1,000). Nuclei were stained blue with DAPI; Catenin delta 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar: 20 μm.
  • Flow cytometric analysis of Catenin delta 1 expression in H9c2 cells using Catenin delta 1 antibody (Cat#63468, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Catenin delta 1.
  • Validation of Catenin delta 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Catenin delta 1 antibody (Cat#63468, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CTNND1
  • Catalog：
    63468
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using catenin delta 1 antibody (Cat#63468). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with catenin delta 1 antibody (Cat#63468, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Western blotting analysis using catenin delta 1 antibody (Cat#63468). Catenin delta 1 expression in wild type (WT) and catenin delta 1 (CTNND1) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with catenin delta 1 antibody (Cat#63468, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using Catenin delta 1 antibody (Cat#63468, 1:1,000), Top panel: wild-type (WT); Bottom panal: Catenin delta 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Catenin delta 1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of H9C2 cells with Catenin delta 1 antibody(Cat #63468, 1:1,000). Nuclei were stained blue with DAPI; Catenin delta 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar: 20 μm.
Flow cytometric analysis of Catenin delta 1 expression in H9c2 cells using Catenin delta 1 antibody (Cat#63468, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Catenin delta 1.
Validation of Catenin delta 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Catenin delta 1 antibody (Cat#63468, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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