Western blotting analysis using POU2AF1 antibody (Cat#4868). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with POU2AF1 antibody (Cat#4868, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of POU2AF1 expression in Raji cells using POU2AF1 antibody (Cat#4868, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, POU2AF1.
POU2AF1; POU Class 2 Homeobox Associating Factor 1; OCA-B; OBF1; BOB1; OCT-Binding Factor 1; POU Domain Class 2-Associating Factor 1; B-Cell-Specific Coactivator OBF-1; POU Class 2 Associating Factor 1; BOB-1; POU Domain Class 2, Associating Factor 1; OBF-1; OCAB
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human POU2AF1
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using POU2AF1 antibody (Cat#4868). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with POU2AF1 antibody (Cat#4868, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of POU2AF1 expression in Raji cells using POU2AF1 antibody (Cat#4868, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, POU2AF1.
Western blotting analysis using POU2AF1 antibody (Cat#4868). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with POU2AF1 antibody (Cat#4868, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of POU2AF1 expression in Raji cells using POU2AF1 antibody (Cat#4868, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, POU2AF1.
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
