Western blotting analysis using SMURF2 antibody (Cat#63756). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMURF2 antibody (Cat#63756, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). SMURF2, SMAD specific E3 ubiquitin protein ligase 2.
Western blotting analysis using SMURF2 antibody (Cat#63756). SMURF2 expression in wild-type (WT) and SMURF2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with SMURF2 antibody (Cat#63756, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of SMURF2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with SMURF2 antibody (Cat#63756, 1:2,000) and analyzed using BD flow cytometer.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human Smurf2
Isotype
Mouse IgG1 kappa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using SMURF2 antibody (Cat#63756). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMURF2 antibody (Cat#63756, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). SMURF2, SMAD specific E3 ubiquitin protein ligase 2.
Western blotting analysis using SMURF2 antibody (Cat#63756). SMURF2 expression in wild-type (WT) and SMURF2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with SMURF2 antibody (Cat#63756, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of SMURF2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with SMURF2 antibody (Cat#63756, 1:2,000) and analyzed using BD flow cytometer.
Western blotting analysis using SMURF2 antibody (Cat#63756). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMURF2 antibody (Cat#63756, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). SMURF2, SMAD specific E3 ubiquitin protein ligase 2.
Western blotting analysis using SMURF2 antibody (Cat#63756). SMURF2 expression in wild-type (WT) and SMURF2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with SMURF2 antibody (Cat#63756, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of SMURF2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with SMURF2 antibody (Cat#63756, 1:2,000) and analyzed using BD flow cytometer.
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