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Home>Product>Primary Antibody

DOHH Recombinant Rabbit mAb

#4892

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Recombinant Antibody
Western blotting analysis using deoxyhypusine hydroxylase antibody (Cat#4892). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with deoxyhypusine hydroxylase antibody (Cat#4892, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Deoxyhypusine hydroxylase expression in HepG2 cells using Deoxyhypusine hydroxylase antibody (Cat#4892, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Deoxyhypusine hydroxylase.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DOHH
  • Catalog：
    4892
  • Application：
    WB, FCM
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name DOHH Recombinant Rabbit mAb
Aliases DOHH; Deoxyhypusine Hydroxylase; HLRC1; HEAT-Like (PBS Lyase) Repeat Containing 1; Deoxyhypusine Hydroxylase/Monooxygenase; HEAT-Like Repeat-Containing Protein 1; Deoxyhypusine Monooxygenase; Deoxyhypusine Dioxygenase; EC 1.14.99.29; MGC4293; HDOHH; NEDMVIC
Background

Gene Name: DOHH

NCBI Gene Entry: 83475

UniProt Entry: Q9BU89

Application Information

Molecular Weight: Predicted, 33 kDa; observed, 33 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB13620

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human DOHH
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using deoxyhypusine hydroxylase antibody (Cat#4892). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with deoxyhypusine hydroxylase antibody (Cat#4892, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Deoxyhypusine hydroxylase expression in HepG2 cells using Deoxyhypusine hydroxylase antibody (Cat#4892, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Deoxyhypusine hydroxylase.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DOHH
  • Catalog：
    4892
  • Application：
    WB, FCM
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using deoxyhypusine hydroxylase antibody (Cat#4892). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with deoxyhypusine hydroxylase antibody (Cat#4892, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Deoxyhypusine hydroxylase expression in HepG2 cells using Deoxyhypusine hydroxylase antibody (Cat#4892, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Deoxyhypusine hydroxylase.
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