Western blotting analysis using BCAR1 antibody (Cat#5010). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCAR1 antibody (Cat#5010, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
BCAR1; BCAR1 Scaffold Protein, Cas Family Member; CASS1; CAS; Cas Scaffolding Protein Family Member 1; P130Cas; Crkas; Breast Cancer Anti-Estrogen Resistance Protein 1; BCAR1, Cas Family Scaffolding Protein; BCAR1, Cas Family Scaffold Protein; Breast Cancer Anti-Estrogen Resistance 1; Crk-Associated Substrate P130Cas; Crk-Associated Substrate; CRK-Associated Substrate; P130cas; CRKAS; CAS1
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500
Note
This product is for research use only.
Data
Western blotting analysis using BCAR1 antibody (Cat#5010). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCAR1 antibody (Cat#5010, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using BCAR1 antibody (Cat#5010). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCAR1 antibody (Cat#5010, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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