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UHRF2 Mouse Oligoclonal Antibody

#5156

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Western blotting analysis using UHRF2 antibody (Cat#5156). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UHRF2 antibody (Cat#5156, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of Hela cells with UHRF2 protein antibody (Cat#5156, 1:1,000). Nuclei were stained blue with DAPI; UHRF2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of UHRF2 expression in HeLa cells using UHRF2 antibody (Cat#5156, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, UHRF2.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    UHRF2
  • Catalog:
    5156
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M
Information
Product Name UHRF2 Mouse Oligoclonal Antibody
Aliases UHRF2; Ubiquitin Like With PHD And Ring Finger Domains 2; RNF107; NIRF; TDRD23; URF2; Ubiquitin-Like With PHD And Ring Finger Domains 2, E3 Ubiquitin Protein Ligase; Ubiquitin-Like-Containing PHD And RING Finger Domains Protein 2; Ubiquitin-Like PHD And RING Finger Domain-Containing Protein 2; RING-Type E3 Ubiquitin Transferase UHRF2; Np95/ICBP90-Like RING Finger Protein; E3 Ubiquitin-Protein Ligase UHRF2; Np95-Like Ring Finger Protein; RING Finger Protein 107; Nuclear Protein 97; MGC33463; Ubiquitin-Like, Containing PHD And RING Finger Domains, 2; Nuclear Zinc Finger Protein NP97; Nuclear Zinc Finger Protein Np97; Np95-Like RING Finger Protein; EC 2.3.2.27; EC 6.3.2
Background

Gene Name: UHRF2

NCBI Gene Entry: 115426

UniProt Entry: Q96PU4

Application Information

Molecular Weight: Predicted, 90 kDa; observed, 90 kDa

Clonality: Mouse oligoclonal antibody

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen Recombinant protein of human UHRF2
Isotype Mouse IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using UHRF2 antibody (Cat#5156). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UHRF2 antibody (Cat#5156, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of Hela cells with UHRF2 protein antibody (Cat#5156, 1:1,000). Nuclei were stained blue with DAPI; UHRF2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of UHRF2 expression in HeLa cells using UHRF2 antibody (Cat#5156, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, UHRF2.
Western blotting analysis using UHRF2 antibody (Cat#5156). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UHRF2 antibody (Cat#5156, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of Hela cells with UHRF2 protein antibody (Cat#5156, 1:1,000). Nuclei were stained blue with DAPI; UHRF2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of UHRF2 expression in HeLa cells using UHRF2 antibody (Cat#5156, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, UHRF2.
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