Western blotting analysis using epidermal growth factor receptor antibody (Cat#H1119). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with epidermal growth factor receptor antibody (Cat#H1119, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using epidermal growth factor receptor antibody (Cat#H1119). Epidermal growth factor receptor expression in wild type (WT) and epidermal growth factor receptor (EGFR) shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with epidermal growth factor receptor antibody (Cat#H1119, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Epidermal growth factor receptor expression in A549 cells using Epidermal growth factor receptor antibody (Cat#H1119, 1:2,000). Green, isotype control; red, Epidermal growth factor receptor.
Immunocytochemical staining shows positive staining in paraffin-embedded human colon. EGFR antibody (Cat#H1119) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human esophagus. EGFR antibody (Cat#H1119) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
