Western blotting analysis using GBE1 antibody (Cat#64802). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GBE1 antibody (Cat#64802, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using GBE1 antibody (Cat#64802). GBE1 expression in wild-type (WT) and GBE1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with GBE1 antibody (Cat#64802, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using GBE1 antibody (Cat#64802, 1:1,000), Top panel: wild-type (WT); Bottom panal: GBE1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; GBE1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Flow cytometric analysis of GBE1 expression in HepG2 cells using GBE1 antibody (Cat#64802, 1:1,000). Green, isotype control; red, GBE1.
